Visual detection of H3 subtype avian influenza viruses by reverse transcription loop-mediated isothermal amplification assay
نویسندگان
چکیده
منابع مشابه
Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus
A novel influenza A (H7N9) virus has emerged in China. To rapidly detect this virus from clinical samples, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the detection of the H7N9 virus. The minimum detection limit of the RT-LAMP assay was 0.01 PFU H7N9 virus, making this method 100-fold more sensitive to the detection of the H7N9 virus than con...
متن کاملA Reverse transcription-PCR assay for detection of type A influenza virus and differentiation of avian H7 subtype
Abstract : Avian influenza virus (AIV) infection is a major cause of influenza mortality in birds and can cause human mortality and morbidity. Although the risk of infection with avian influenza virus (AIV) is generally low for most people, the pathogenic virus can cross the species barrier and acquires the ability to infect and be transmitted among the human population; therefore the ra...
متن کاملDetection of dengue viruses using reverse transcription-loop-mediated isothermal amplification
BACKGROUND Early and rapid detection of dengue virus (DENV) infection during the febrile period is crucial for proper patient management and prevention of disease spread. An easy to perform and highly sensitive method is needed for routine implementation especially in the resource-limited rural healthcare settings where dengue is endemic. METHODS A single-tube reverse transcription-loop-media...
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ژورنال
عنوان ژورنال: Virology Journal
سال: 2011
ISSN: 1743-422X
DOI: 10.1186/1743-422x-8-337